2024/02/07 09:03:24 Running on Python 3.8.2 (default, Apr 23 2020, 14:22:33) [GCC 8.3.0]. 2024/02/07 09:03:24 Loading a config file from /dfast_core/example/dfc_config/dfast_web_config.py 2024/02/07 09:03:24 DFAST_DB_ROOT is specified [/dfast_core_db] 2024/02/07 09:03:24 OS type is Linux. 2024/02/07 09:03:24 Adding /dfast_core/bin/Linux to PATH. 2024/02/07 09:03:24 DFAST pipeline started. (version 1.2.20) 2024/02/07 09:03:24 Results will be generated into '/dfast_volumes/jobs/7d52e3dd-145e-44ac-b9fe-4d0d441098e9/annotation'. 2024/02/07 09:03:24 Loading a genomic fasta file from /dfast_volumes/jobs/7d52e3dd-145e-44ac-b9fe-4d0d441098e9/input/genome.fna 2024/02/07 09:03:24 Genome source information: organism=, strain= 2024/02/07 09:03:24 The query genome is treated as a draft genome with 3 sequences. 2024/02/07 09:03:24 Sequences shorter than 200 will be eliminated. 2024/02/07 09:03:24 Sequences will be renamed as sequence1, sequence2... 2024/02/07 09:03:24 Locus_tag settings: locus_tag_prefix=LOCUS and step=10. 2024/02/07 09:03:24 Locus_tags are assigned separately to each feature type. e.g. CDS: LOCUS_000xx, rRNA: LOCUS_r000xx, tRNA: LOCUS_t000xx, tmRNA: LOCUS_tm000xx. 2024/02/07 09:03:24 Remove_Partial_Feature is enabled. 2024/02/07 09:03:24 Remove_Overlapping_Feature is enabled. Priority: ['assembly_gap', 'CRISPR', ('tmRNA', 'tRNA', 'rRNA'), 'CDS'] 2024/02/07 09:03:24 Initializing structural annotation tools... 2024/02/07 09:03:24 GAPannotator initialized. (Version 1.0) 2024/02/07 09:03:24 Setting GAPannotator options. {'len_cutoff': 5, 'linkage_evidence': 'paired-ends', 'gap_type': 'within scaffold'} 2024/02/07 09:03:24 Checking MetaGeneAnnotator... 2024/02/07 09:03:24 MetaGeneAnnotator initialized. (Version 2008/08/19) 2024/02/07 09:03:24 Setting MetaGeneAnnotator options. {'cmd_options': '-s', 'transl_table': 11} 2024/02/07 09:03:24 Checking Aragorn version... 2024/02/07 09:03:24 Aragorn initialized. (Version 1.2.38) 2024/02/07 09:03:24 Setting Aragorn options. {'gcode': '-gcbact', 'cmd_options': '-l', 'transl_table': 11} 2024/02/07 09:03:24 Checking Barrnap version... 2024/02/07 09:03:24 Barrnap initialized. (Version 0.8) 2024/02/07 09:03:24 Checking CRT version... 2024/02/07 09:03:24 CRT initialized. (Version 1.2) 2024/02/07 09:03:24 Setting CRT options. {'jar_file': '/dfast_core/bin/common/CRT1.2-CLI.jar', 'java_options': '', 'cmd_options': ''} 2024/02/07 09:03:24 Checking Java version... 2024/02/07 09:03:24 Java initialized. (Version 11.0.9.1) 2024/02/07 09:03:24 Initializing annotation components... 2024/02/07 09:03:24 DBsearch initialized. 2024/02/07 09:03:24 Setting DBsearch options. {'cpu': 3, 'skipAnnotatedFeatures': True, 'evalue_cutoff': 1e-06, 'qcov_cutoff': 75.0, 'scov_cutoff': 75.0, 'pident_cutoff': 0.0, 'aligner': 'ghostx', 'aligner_options': {}, 'database': '/dfast_core_db/protein/DFAST-default.ref', 'db_name': ''} 2024/02/07 09:03:24 Checking GHOSTX version... 2024/02/07 09:03:24 GHOSTX initialized. (Version 1.3.6) 2024/02/07 09:03:26 Reference DB loaded. 381721 sequences. [version=1.2.0, dbname=DFAST-default, contributor=yt,ddbj, modified=20230404] 2024/02/07 09:03:26 PseudoGeneDetection initialized. 2024/02/07 09:03:26 Setting PseudoGeneDetection options. {'cpu': 1, 'skipAnnotatedFeatures': False, 'extension': 300, 'scov_cutoff': 85, 'transl_table': 11} 2024/02/07 09:03:26 Checking lastdb version... 2024/02/07 09:03:26 lastdb initialized. (Version 959) 2024/02/07 09:03:26 Setting lastdb options. {'cpu': 1, 'skipAnnotatedFeatures': False, 'extension': 300, 'scov_cutoff': 85, 'transl_table': 11} 2024/02/07 09:03:26 Checking lastal version... 2024/02/07 09:03:26 lastal initialized. (Version 959) 2024/02/07 09:03:26 Setting lastal options. {'cpu': 1, 'skipAnnotatedFeatures': False, 'extension': 300, 'scov_cutoff': 85, 'transl_table': 11} 2024/02/07 09:03:26 Checking Blastp version... 2024/02/07 09:03:26 Blastp initialized. (Version 2.6.0) 2024/02/07 09:03:26 Start structural annotation process using 3 CPUs 2024/02/07 09:03:29 0 assembly_gap features were detected by GAPannotator. 2024/02/07 09:03:30 2352 CDS features were detected by MetaGeneAnnotator. 2024/02/07 09:03:30 57 tRNA features were detected by Aragorn. 2024/02/07 09:03:30 12 rRNA features were detected by Barrnap. 2024/02/07 09:03:30 1 CRISPR (later will be replaced by repeat_region) features were detected by CRT. 2024/02/07 09:03:31 Removed 8 overlapping features. 2024/02/07 09:03:31 Start executing functional annotation components. 2024/02/07 09:03:31 DBsearch will be performed using 3 CPUs. 2024/02/07 09:03:58 DBsearch done 2/3. 2024/02/07 09:04:02 DBsearch done 1/3. 2024/02/07 09:04:03 DBsearch done 3/3. 2024/02/07 09:04:04 Last format DB will be performed using 1 CPUs. 2024/02/07 09:04:04 Last format DB done 1/1. 2024/02/07 09:04:04 Last alignment will be performed using 1 CPUs. 2024/02/07 09:04:05 Last alignment done 1/1. 2024/02/07 09:04:06 19 CDS features were marked as possible pseudo due to internal stop codons. 2024/02/07 09:04:06 20 CDS features were marked as possible pseudo due to frameshift. 2024/02/07 09:04:06 Removed 0 partial features. 2024/02/07 09:04:06 Assigning locus_tags to CDS, rRNA, tRNA, tmRNA 2024/02/07 09:04:06 Setting output verbosity level to 3. 2024/02/07 09:04:06 Writing a genome fasta file to /dfast_volumes/jobs/7d52e3dd-145e-44ac-b9fe-4d0d441098e9/annotation/genome.fna 2024/02/07 09:04:06 Writing a CDS fasta file to /dfast_volumes/jobs/7d52e3dd-145e-44ac-b9fe-4d0d441098e9/annotation/cds.fna 2024/02/07 09:04:06 Writing a protein fasta file to /dfast_volumes/jobs/7d52e3dd-145e-44ac-b9fe-4d0d441098e9/annotation/protein.faa 2024/02/07 09:04:06 Writing an RNA fasta file to /dfast_volumes/jobs/7d52e3dd-145e-44ac-b9fe-4d0d441098e9/annotation/rna.fna 2024/02/07 09:04:06 Writing a GFF file to /dfast_volumes/jobs/7d52e3dd-145e-44ac-b9fe-4d0d441098e9/annotation/genome.gff 2024/02/07 09:04:06 Writing a GenBank format file to /dfast_volumes/jobs/7d52e3dd-145e-44ac-b9fe-4d0d441098e9/annotation/genome.gbk 2024/02/07 09:04:07 Writing an EMBL format file to /dfast_volumes/jobs/7d52e3dd-145e-44ac-b9fe-4d0d441098e9/annotation/genome.embl 2024/02/07 09:04:07 Writing a DDBJ annotation file to /dfast_volumes/jobs/7d52e3dd-145e-44ac-b9fe-4d0d441098e9/annotation/ddbj/mss.ann 2024/02/07 09:04:07 Writing a DDBJ sequence file to /dfast_volumes/jobs/7d52e3dd-145e-44ac-b9fe-4d0d441098e9/annotation/ddbj/mss.fasta 2024/02/07 09:04:08 'center_name' is not specified. 'my_center' is used tentatively. 2024/02/07 09:04:08 Writing a GenBank tbl file to /dfast_volumes/jobs/7d52e3dd-145e-44ac-b9fe-4d0d441098e9/annotation/genbank/genbank.tbl 2024/02/07 09:04:08 Writing a GenBank fsa file to /dfast_volumes/jobs/7d52e3dd-145e-44ac-b9fe-4d0d441098e9/annotation/genbank/genbank.fsa 2024/02/07 09:04:09 DFAST pipeline completed! 2024/02/07 09:04:09 Total running time: 0h0m45s