DDBJ Fast Annotation and Submission Tool (DFAST) is a bacterial genome annotation pipeline integrated with quality and taxonomy assessment methods. DFAST is developed so that all the procedure required for submission can be done seamlessly on-line, thus it can be used as an on-line workspace to prepare submission files to DDBJ Mass Submission System (MSS).

DAGA (DFAST Archive of Genome Annotation) stores genomes obtained from DDBJ/ENA/GenBank and SRA with consistent annotation and assessment using DFAST. Currently, DAGA provides annotated genomes for Lactic Acid Bacteria (Lactobacillus and Pedioccoccus).

Contact

This web site is operated by Genome Informatics Laboratory at the National Institute of Genetics.

If you have any question, request, or suggestion, please contact the E-mail address below.

dfast [at] nig.ac.jp

Citation

If you use our web service, please cite either of the following:

Tanizawa Y, Fujisawa T, Kaminuma E, Nakamura Y, and Arita M

DFAST and DAGA: web-based integrated genome annotation tools and resources

Bioscience of Microbiota, Food and Health, 35(4) pp. 173-184, 2016

Full paper is available at J-Stage.

Tanizawa Y, Fujisawa T, and Nakamura Y

DFAST: a flexible prokaryotic genome annotation pipeline for faster genome publication.

Bioinformatics, 34(6) 1037–1039, 2018.

Disclaimer

While we make effort to ensure the accuracy and reliability of the software and data,

we do not warrant or assume any legal liability or responsibility for the data and the result available at this site.

The version numbers shown here denote those for the web service (not for the analytical pipeline).
It is recommendable to refer to the version number of the analytical pipeline in your papre, which can be found in the log file.

v. 1.4.0 (2021.2.22)

DFAST web service has moved to NIG-SC.

v. 1.3.0 (2020.7.7)

DFAST Quality Control (DFAST_QC) become available.

v. 1.2.4 (2019.11.7)

Reference database updated (1.1.5) for Staphylococcus.

v. 1.1.0 (2018.11.7)

Genetic code 4 (Mycoplasma/Spiroplasma) supported.

v. 1.0.2 (2018.8.15)

Prodigal (v2.6.3) and tRNAscan-SE (v2.0) have become available in the structural annotation phase.

v. 1.0.1 (2018.5.15)

Minor updates.
Fixed typos in the DFAST default reference database.
Suppressed output for ambiguous tRNA.

v. 1.0.0 (2017.8.10)

Replaced the background annotation engine of the DFAST web service with our original pipeline called DFAST-core.
The older pipeline based on Prokka is now refered to as a legacy server.

v. 0.9.3 (2017.7.1)

Web API has become available.

Reference database for Bifidobacterium has become available.

v. 0.9.2 (2017.5.1)

General purpose reference databases (RefSeq and its subsets for Firmicutes, Actinobacteria, Proteobacteria) have become available.

DFAST has become able to generate DDBJ submission files for complete genomes by specifying additional information such as sequence names and topologies.

Signal peptide prediction (signalP) has become disabled by default. To enable this, check the box in the job submission form. (Signal peptide prediction will not be conducted if you choose RefSeq for a reference database.)

v. 0.9.1 (2016.3.1)

DFAST reference database for lactic acid bacteria is mainly constructed based on the complege genomes of Lactobacillaceae (Lactobacillus and Pediococcus) and representative genomes of other family (Leuconostocaceae, Enterococcaceae, Streptococcaceae).

Annotation for organisms other than Lactobacillaceae is not fully supported.