× [Notice] 2026.10.06 DFAST has been updated to ver. 1.5.1. The reference databases for functional annotation have been updated to COG v2.0 (NCBI CDD 3.21) and NCBIfam 20.0, the latter replacing TIGRFAMs 15.0. In addition, rRNA prediction is now performed by our own implementation of the Barrnap method, which runs nhmmer directly against the Barrnap 0.9 profile models. Annotation results may therefore differ slightly from those obtained with earlier versions.