× [Notice] 2026.11.06 DFAST has been updated to ver. 1.6.0. CDS prediction now uses Prodigal by default (MetaGeneAnnotator can still be selected), CRISPR prediction uses Diced with the parameters of CRT, and rRNA prediction uses the Rfam models of bacteria and archaea, so annotation results may differ from those of earlier versions. Accessory annotations can now be selected individually: antimicrobial resistance genes (CARD), virulence factors (VFDB), plasmid replicons (PlasmidFinder), mobile genetic elements (MobileElementFinder), and newly integrons (IntegronFinder) and anti-phage defense systems (DefenseFinder). CARD/VFDB reference genes are now mapped onto the genome, and the results are summarized in accessory_summary.tsv. The maximum size of a query file is now 20 MB.