DFAST is an automatic annotation service for prokaryotic genomes. It also assists data submission to DDBJ.
Who can use DFAST？
DFAST is freely available for both academic and for-profit users. No user registration is required.
You can also log-in to the DFAST web service with your D-way account. When logged-in, you can browse your submitted jobs in the Job History page.
How can I use DFAST？
Just upload your genome sequence data in a FASTA format. Either single- or multi-FASTA file is OK.
DFAST generates annotation results in standard annotation formats such as FASTA, GenBank, and GFF as well as in a DDBJ data submission format.
If I use DFAST, is the result automatically going to be submitted to DDBJ or is the data submission to DDBJ mandatory？
No. To submit your data to DDBJ, you need to send files generated by DFAST by yourself. You can also use DFAST only to annotate your genome without submitting the result to DDBJ.
What can I do when logged-in with a D-way user account？
The Job History page becomes available, where you can browse all the jobs executed while you are logged-in. Bulk data downloading for multiple jobs is also available. You can import the job to the history by specifying the job ID.
You can apply a D-way user account from here.
How is the data confidentiality protected？
A 128-bit unique ID (UUID) will be assigned to the submitted job. Only users who know the ID can access the result.
What kinds of personal information are collected by DFAST？
For security purposes, job submitters' IP addresses are recorded. If provided, job titles and email addresses are collected. We use Google Analytics to survey system usage. Each access information will not be disclosed, but statistics such as annual numbers of processed jobs or visitors might be disclosed.
How long are the results kept?
All the data will be deleted after 30 days have passed since your last access to the result. You can also delete the data immediately by pressing the 'Delete' button in the result page.
I got an error.
Please check the query file. In particular, ambiguous nucleotides such as R (=A or G) or Y (=T or C) are not allowed, which should be replaced with 'N'. Use this script to replace them replace_ambiguous.py.